Affinity purification of cytosolic epoxide hydrolase from human, rhesus monkey, baboon, rabbit, rat and mouse liver.
نویسندگان
چکیده
An affinity purification system based on elution of cytosolic epoxide hydrolase from a methoxycitronellyl thiol ligand with 4-azidochalcone oxide was applied to a variety of samples including liver from human, monkey, baboon, rabbit, rat and mouse as well as mammary gland from mouse. Hepatic tissues yielded a major 58 kDa band on SDS-PAGE, but the system had to be modified slightly to remove a 33 kDa band for rat. All of the affinity purified hydrolases showed similar properties with regard to substrate selectivity, pH dependence and mobilities on SDS-PAGE.
منابع مشابه
Microsomal and cytosolic epoxide hydrolases in rhesus monkey liver, and in normal and neoplastic human liver.
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عنوان ژورنال:
- Comparative biochemistry and physiology. B, Comparative biochemistry
دوره 87 1 شماره
صفحات -
تاریخ انتشار 1987